Rabbit anti‑Bovine ALPI / Alkaline Phosphatase Polyclonal RW-C59289-2
Antibody: ALPI / Alkaline Phosphatase Rabbit anti-Bovine Polyclonal Antibody, Application: WB, ELISA, Reactivity: Bovine, Format: Unconjugated, Unmodified, Descriptio: Alkaline Phosphatase antibody RW-C59289 is an unconjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA and WB., Targe: Bovine ALPI / Alkaline Phosphatase, Synonym: ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP, Hos: Rabbit, Reactivit: Bovine (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Purificatio: Delipidated and defibrinated, Modification: Unmodified, Immunoge: Alkaline Phosphatase [Calf intestine]., Specificit: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Calf intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. No reaction was observed against the bacterial form of the enzyme., Application: Western blot (1:500 - 1:2000)
ELISA (1:4000 - 1:20000), Usag: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. RW-C60884 and ABTS as a substrate for 30 minutes at room temperature., Presentatio: Lyophilized from 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, Reconstitutio: Reconstitute in 2 mL of deionized water., Storag: Store at 4°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ALPI / Alkaline Phosphatas: Uniprot: P09923 NCBI: NM_001631 NP_001622.2