Rabbit anti‑Bovine IgG Fab'2 IgG Polyclonal RW-C60201-1

Antibody: Bovine IgG Fab'2 Rabbit anti-Bovine Polyclonal (AP) Antibody, Application: IHC, WB, ELISA, Reactivity: Bovine, Format: Alkaline Phosphatase, Unmodified, Descriptio: Bovine IgG Fab'2 antibody RW-C60201 is an AP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB., Targe: Bovine IgG Fab'2, Hos: Rabbit, Reactivit: Bovine (tested or 100% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Alkaline Phosphatase, Purificatio: Affinity chromatography, Modification: Unmodified, Immunoge: Bovine IgG F(ab')2 fragment., Specificit: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum. No reaction was observed against Bovine IgG F(c)., Application: IHC (1:200 - 1:1000)
Western blot (1:500 - 1:2500)
ELISA (1:10000 - 1:20000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-anti body based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested., Presentatio: 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol, Storag: Store at 4°C. Do not freeze., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.