Rabbit anti‑Bovine CA2 / Carbonic Anhydrase II IgG Polyclonal RW-C209667-100
Antibody: CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Lyophilized) (Biotin) Antibody, Application: WB, ELISA, Reactivity: Bovine, Format: Biotin, Lyophilized, Descriptio: Carbonic Anhydrase II antibody RW-C209667 is a biotin-conjugated rabbit polyclonal antibody to bovine Carbonic Anhydrase II (CA2). Validated for ELISA and WB., Targe: Bovine CA2 / Carbonic Anhydrase II, Synonym: CA2 | CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | Carbonic anhydrase 2 | Carbonic anhydrase B, Hos: Rabbit, Reactivit: Bovine (tested or 100% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Biotin, Purificatio: Delipidation, salt fractionation and ion exchange chromatography followed by dialysis., Modification: Lyophilized, Immunoge: Carbonic Anhydrase II [Bovine Erythrocytes]., Specificit: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other sources may occur but has not been specifically determined., Application: Western blot (1:200 - 1:1000)
ELISA (1:2000 - 1:10000), Usag: Anti-Carbonic Anyhdrase II has been assayed against 1.0 ug of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:20000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested., Presentatio: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, Reconstitutio: Reconstitute in 100 µL of deionized water., Storag: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CA2 / Carbonic Anhydrase I: Uniprot: P00918 NCBI: NM_000067 NP_000058.1