Mouse anti‑Rat CD163 IgG1 Monoclonal RW-C188455-0.1

Antibody: CD163 Mouse anti-Rat Monoclonal (ED2) Antibody, Application: IHC, IHC-P, IHC-Fr, IF, WB, IP, Reactivity: Rat, Format: Unconjugated, Unmodified, Descriptio: CD163 antibody RW-C188455 is an unconjugated mouse monoclonal antibody to rat CD163. Validated for IF, IHC, IP and WB., Targe: Rat CD163, Synonym: CD163 | CD163 antigen | Macrophage-associated antigen | M130 | CD163 molecule | Hemoglobin scavenger receptor | MM130, Hos: Mouse, Reactivit: Rat (tested or 100% immunogen sequence identity), Clonalit: IgG1 Monoclonal, Clon: ED2, Conjugation: Unconjugated, Purificatio: Protein A purified, Modification: Unmodified, Immunoge: Rat spleen cell homogenate., Specificit: Recognizes the rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.* This clone may be used in histology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to ref. Whiteland et al., Application: IHC
IHC - Paraffin
IHC - Frozen (1:50 - 1:100)
Immunofluorescence
Western blot
Immunoprecipitation, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Presentatio: PBS, 0.09% Sodium Azide, Storag: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About CD16: Uniprot: Q86VB7 NCBI: NM_004244 NP_004235.4