Mouse anti‑Rat CD3 IgM Monoclonal RW-C58677-0.1

Antibody: CD3 Mouse anti-Rat Monoclonal (1F4) Antibody, Application: IHC, IHC-P, IHC-Fr, IP, Flo, Reactivity: Rat, Format: Unconjugated, Unmodified, Other formats: FITC, Descriptio: CD3 antibody RW-C58677 is an unconjugated mouse monoclonal antibody to rat CD3. Validated for Flow, IHC and IP., Targe: Rat CD3, Hos: Mouse, Reactivit: Rat (tested or 100% immunogen sequence identity), Clonalit: IgM Monoclonal, Clon: 1F4, Conjugation: Unconjugated. Also available conjugated with FITC., Purificatio: Ammonium sulfate precipitation, Modification: Unmodified, Immunoge: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore., Specificit: Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA., Application: IHC
IHC - Paraffin (1:10)
IHC - Frozen (1:10 - 1:25)
Immunoprecipitation
Flow Cytometry (1:10 - 1:25), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request., Presentatio: PBS, 0.09% Sodium Azide, Storag: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.